Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations
نویسندگان
چکیده
منابع مشابه
Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations
Detection of low-level DNA variations in the presence of wild-type DNA is important in several fields of medicine, including cancer, prenatal diagnosis and infectious diseases. PCR-based methods to enrich mutations during amplification have limited multiplexing capability, are mostly restricted to known mutations and are prone to polymerase or mis-priming errors. Here, we present Differential S...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2012
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gks1250